Additional file 10. Comparison of CYP4 protein sequences

Maximum parsimony analysis identified one CYP4V10 gene with two putative allelic variants (CYP4V10v1 and CYP4V10v2) that share 98.8% amino acid identity (Fig. 4, part A) but differ by a five amino acid insertion near their C-terminus end. Five of the 65 clones within the CYP4V10 subfamily also contained a 68 bp deletion causing a frame shift, resulting in a premature stop codon and a loss of 155 amino acids, likely resulting in a nonfunctional P450 protein lacking its heme-binding region.